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KMID : 0363320220430010068
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Journal of Korean Oriental Internal Medicine
2022 Volume.43 No. 1 p.68 ~ p.78
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Inhibitory Effect of Rhododendron Mucronulatum Root Extract on Allergic Inflammation
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Jang Si-Sung
Lee dae-Joong
Song Ji-Hoon
Park Do-Hwi
Jeon Chan-Yong
Hwang Gwi-Seo
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Abstract
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Objective: In this study, we investigated the protective effect of rhododendron mucronulatum extract (RME) on allergic reactions and inflammation.
Methods: The effect of RME was determined using ELISA and RT-PCR in RBL-2H3 mast cells and RAW 264.7 macrophage cells. We determined cell viability, β-hexosaminidase release, and the synthesis of IL-4 and TNF-α in RBL-2H3 cells. In addition, we determined NO from RAW 264.7 and the gene expression of IL-1β, iNOS, IL-6, TNF-α, and IL-10.
Results: RME inhibited β-hexosaminidase release and synthesis of IL-4 and TNF-α in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. In addition, RME inhibited the production of NO and the gene expression of IL-1β, iNOS, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells.
Conclusion: From these results, we concluded that RME possesses anti-allergic activity and anti-inflammatory activity due to the inhibition of mast cells and macrophage function.
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KEYWORD
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Rhododendron Mucronulatum, allergy, β-hexosaminidase, interleukin, TNF-α, NO
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